1. Microbiology & Food Hygiene

Genetic analysis of resistance to detergents and industrial cleaning solutions in Listeria monocytogenes growing as planktonic cells and biofilms

Project Reference:

03-PG-09

Status:

Completed

Commencement Date:

October, 2003

Project Duration:

36 months

Abstract:

Investigation of several outbreaks of listeriosis worldwide has indicated that contamination of foods with this pathogen appears to have occurred from a persistent source of the pathogen in the food manufacturing plant that survived industrial cleaning protocols.

The objective of this project was to identify genes involved in the resistance of planktonic Listeria monocytogenes cells to detergents and industrial cleaning agents. Mutants exhibiting reduced detergent resistance were subsequently analysed for detergent resistance in biofilms. 

In addition the correlation between detergent and bile resistance were investigated to test the hypothesis that industrial detergents may select for bile resistant strains of L. monocytogenes in the food-processing environment.

 

Principal Contractor(s):

Dr Cormac Gahan, University College Cork

Collaborator(s):

Single supplier

Outputs:

Report:

The PhD thesis is available on request and can be accessed through University College Cork

Peer review:

Ryan E, Gahan CGM and C Hill. 2008. A significant role for Sigma B in the detergent stress response of Listeria monocytogenes. Letters in Applied Microbiology. 46(2):148-54