1. Microbiology & Food Hygiene

Detection and molecular characterization of selected pathogenic organisms isolated from unpasteurised milk using milk filters

Project Reference:

00-RESR-046

Status:

Completed

Commencement Date:

February, 2001

Project Duration:

36 months

Abstract:

Surveys have shown that a substantial proportion of farm families in the Republic of Ireland consume raw milk. This practice is inadvisable because of the risk of pathogenic microorganisms being present in the milk.

As well as completing a survey of raw milk consumption by farm families, this study determined baseline values for the incidence of a number of pathogens including Mycobacterium avium subsp. paratuberculosis (MAP), Verocytotoxigenic Escherichia coli (VTEC), and Salmonella species in unpasteurised milk in commercial dairy herds in the Cork region of Ireland. 

 

Principal Contractor(s):

Mr Jim Buckley, Cork County Council

Collaborator(s):

Prof Seamus Fanning, University College Dublin

Dr Michael Rowe, Queen’s University Belfast

Outputs:

Report:

No published report

Peer review:

Reen, J., Boyd, E.F., Porwillik, S., Murphy, B.P., Gilroy, D., Fanning, S. and McClelland, M. (2005) Genomic comparisons of Salmonella enterica Serovar Dublin, Agona and Typhimurium strains recently isolated from milk filters and bovine samples from Ireland, using a Salmonella microarray. Applied and Environmental Microbiology. 71: (3) 1616-1625.

Brenda P. Murphy, Mary Murphy, James F. Buckley, Deirdre Gilroy, Michael T. Rowe, David McCleery, and Séamus Fanning.  (2005) In-line milk filter analysis: Escherichia coli O157 surveillance of milk production holdings. International Journal of Hygiene and Environmental Health 208: 407-413.

Brenda P. Murphy, Rebecca O’Mahony, James F. Buckley, Priscilla Shine, E. Fidelma Boyd, Deirdre Gilroy, and Séamus Fanning (2007). Investigation of a large collection of non-typhoidal Salmonella of various serotypes cultured between 1953 and 2004 for the presence class 1 integron gene cassette.  FEMS Microbiol Lett. 266: 170-176. 

Brenda P. Murphy, James F. Buckley, Elizabeth M. O’Connor, Deirdre Gilroy, and Séamus Fanning (2008). Comparison of Salmonella species recovered from Irish liquid milk production holdings with temporal clinical veterinary isolates.  International Journal of Hygiene and Environmental Health, 211: 283-291.

Brenda P. Murphy, Emer O’Mahony, James F. Buckley, Stephen O’Brien and Séamus Fanning (2009). Characterisation of Staphylococcus aureus isolated from dairy animals in Ireland, Zoonoses and Public Health (Published online Mar 2nd 2009 in advance of print).

Brenda P. Murphy, Emer O’Mahony, James F. Buckley and Séamus Fanning (2009). Characterisation of a small collection of Listeria monocytogenes isolated during surveillance of dairy production holdings supplying the farmhouse cheese sector in Ireland. Journal of Food Protection, (In Preparation).

Brenda P. Murphy, Emer O’Mahony, Noelle McCarthy, James F. Buckley and Séamus Fanning (2009). Campylobacter and Salmonella species: emerging pathogen surveillance of liquid dairy holdings supplying the farmhouse cheese sector in Ireland. International Journal of Food Microbiology, (In Preparation).